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2. Preparation of Adaptor Ligated DNA 2.1. Add the following to the microfuge tube: For 10 ng COMPONENT VOLUME (red) T4 DNA Ligase Buffer for Ion Torrent 4 µl Sterile H 2O 9 µl (red) NEBNext DNA Library Adaptors for Ion Torrent 2 µl (red) Bst 2.0 WarmStart DNA Polymerase 1 µl (red) T4 DNA Ligase 4 µl Total Volume 20 µl For 100 ng– 1 µg
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